Planet, Columbia University, United States of America parasitica within the Rhizobiaceae, as sister to a group containing Agrobacterium and Rhizobium species.Ĭitation: Kvist S, Narechania A, Oceguera-Figueroa A, Fuks B, Siddall ME (2011) Phylogenomics of Reichenowia parasitica, an Alphaproteobacterial Endosymbiont of the Freshwater Leech Placobdella parasitica. The phylogenetic analyses support the nesting of R. parasitica interacts with its host in part by transmembrane signaling and that several of its genes show orthology across Rhizobiaceae. parasitica possesses genes coding for proteins related to nitrogen fixation, iron/vitamin B translocation and plasmid survival. A total of 358 orthologous genes were used for the phylogenetic analyses.
Further, amino acid sequences inferred from the contigs were used as queries against the 50 bacterial genomes to establish orthology. The bacterial contigs were compared to 50 annotated bacterial genomes and the genome of the freshwater leech Helobdella robusta using a BLASTn protocol. We sequenced a combined pool of host and symbiont DNA from 36 pairs of mycetomes and performed an in silico separation of the different DNA pools through subtractive scaffolding. parasitica and on performing a robust phylogenetic analysis, based on more data than previous hypotheses, to test its position among related bacteria. Here, we focused both on exploring the genomic makeup of R. However, the function of the bacterial symbionts has remained unclear. These bacteria are harbored in the epithelial cells of the mycetomal structures of their freshwater leech hosts, Placobdella spp., and these structures have no other obvious function than housing bacterial symbionts. parasitica, are currently the only known mutualistic, alphaproteobacterial endosymbionts to inhabit leeches. Although several commensal alphaproteobacteria form close relationships with plant hosts where they aid in (e.g.,) nitrogen fixation and nodulation, only a few inhabit animal hosts.
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